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identifier: SDY314
description:
Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
aggregation:
instance of dataset
availability:
available with registration
acknowledges:
NIAID (HIPC funded)
isAbout:
1. Compare lymphocyte responses at Days 6-8 and the lymphocyte and serology responses at Day 28 post-immunization following administration of seasonal trivalent inactivated influenza vaccine. 2. Evaluate changes in cytokine profile in the immune response (CD4, CD8, gamma-delta cells, B cell responses) from Day 0 to Day 6-8 for T cells and antibody-secreting cells (ASCs). 3. Evaluate changes in cytokine profile in the immune response (CD4, CD8, gamma-delta cells, B cell, and serology responses) from Day 0 to Day 28 for HAI responses. 4. Evaluate any potential gene expression signatures either at Day 0 or during the early response period Day 6-8 that are associated with immune senescence, potentially providing insight into mechanism. 5. Compare cell specific gene expression differences between age groups for both whole blood and whole PBMC. 6. Determine the effect of cell subset frequency variation on serum cytokine abundance. 7. Evaluate CMV and EBV seroreactivity at Day 0 and additional timepoints throughout the study as able.
subject species: Homo sapiens
study conditions: Influenza
study type: Longitudinal
study category: Vaccine Response
assay type: Flow Cytometry
Gene Expression
HAI
HLA Typing
Neutralizing Antibody
time point:
subject race: American Indian or Alaska Native
Asian
Other
Unknown
White
subject gender: Female
Male
subject age: 21-30
31-40
61-70
> 70
name:
Aging, Immune responses to influenza vaccine.
affiliations:
Stanford University
fullName:
Mark Davis
roles:
principal investigator
name:
T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2008 (See companion studies SDY315 2012 / SDY312 2009 / SDY311 2010 / SDY112 2011)
size:
92
output:
1. Combinatorial tetramer analysis by FACS and/or CyTOF; Phosphoflow. 2. Luminex; Gene expression profiling (Affymetrix microarray). 3. Luminex; Gene expression profiling (Affymetrix microarry); Microneutralization assays. 4. Gene expression profiling (Affymetrix microarray); Immune phenotyping by CyTOF. 5. Gene expression profiling (Affymetrix microarray). 6. Immune phenotyping by CyTOF; Luminex. 7. ELISA
description:
Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
name:
ImmuneSpace
homePage: http://www.immunespace.org

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