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identifier: SDY28
description:
Our broad objective is to examine the role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia vaccine-induced humoral and cell-mediated immune responses among a cohort of 1,000 recently vaccinated subjects.In Research Area 1, we propose to study associations between specific class I and II HLA alleles (HLA-A, -B, -C, -DRB, -DQA, -DQB, -DPA, -DPB), specific cytokine genes, polymorphisms of the above cytokine receptors, a genome-wide SNP analysis; and variations in immune response to smallpox vaccine.In Research Area 2, we focus on identifying associations between expression and function of these same candidate genes likely to regulate immune response variations and humoral and cell-mediated immune responses following smallpox vaccination in selected human subjects. Both gene products (i.e., secreted proteins), cell surface expression, and measures of gene regulation/activation will be pursued. We focus on gene families involved in initiating, sustaining and regulating innate and adaptive immune responses, as well as those directly involved in directing specific antibody and cytotoxic T cell responses.
aggregation:
instance of dataset
availability:
available with registration
acknowledges:
NIAID
primaryPublications: 23594957
23806267
27513748
22949304
19038260
isAbout:
To correlate variability in humoral and cell-mediated immune responses to vaccinia virus immunization with genetic variation in the HLA, cytokine and cytokine receptor genes and the broader genome.
subject species: Homo sapiens
study conditions: Vaccinia
study type: Observational
study category: Vaccine Response
assay type: ELISA
Flow Cytometry
Gene Expression
HLA Typing
time point:
subject race: American Indian or Alaska Native
Black or African American
Other
Unknown
White
subject gender: Female
Male
Not Specified
subject age: 11-20
21-30
31-40
41-50
Unknown
name:
Role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia-induced humoral and cell-mediated immune responses among a cohort of 1,000 recently vaccinated subjects.
affiliations:
Mayo Vaccine Research Group
fullName:
Gregory Poland
roles:
principal investigator
name:
Humoral and Cell-Mediated Immune Responses to Vaccinia Virus Immunization
size:
1092
output:
ELISA-neutralizing antibody titers.ELISPOT-Antigen specific cells.HLA Genotyping- A, B, C, DRB, DQA, DPA, DPB.SNP Genotyping of Cytokine and cytokine receptor genes.Genome Wide Association Study (GWAS).Microarray analysis of PBMC responses.ELISA- Cytokines.Flow Cytometry-HLA Class I & II.
description:
Our broad objective is to examine the role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia vaccine-induced humoral and cell-mediated immune responses among a cohort of 1,000 vaccinated subjects.
name:
ImmuneSpace
homePage: http://www.immunespace.org