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identifier: 1418817
description:
epitope description:LKFKQNVNRPSR
antigen name:Other Fagopyrum esculentum (common buckwheat) protein
host organism:Homo sapiens
antibody name:patient sera
aggregation:
instance of dataset
availability:
available
primaryPublications: 15310064
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1006009
landingPage: http://www.iedb.org/assay/1418817
type:
Literature
publicationVenue:
J Plant Physiol
dates:
2004
study type: b cell assays
subject species: Fagopyrum esculentum
fullName:
Hiroyuki Yoshioka
Tsuyoshi Ohmoto
Atsuo Urisu
Yoshinori Mine
Taiji Adachi
method:
western blot
name:
Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat.
description:
The common buckwheat (F. esculentum Moench) strain BC6F2 was established by 6 backcrosses and one selfing following interspecific hybridization between common buckwheat (cv. Miyazakizairai) and F. homotropicum Ohnishi.
Pooled serum IgE from 8 patients with buckwheat allergy reacted with the epitope by a SPOT assay. The core epitope sequence was deduced to be residues 13-20 (QNVNRPSR) through study of overlapping peptides. Single amino acid substitutions with Ala or Met did not establish critical residues in the sequence.

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