Immunological Data Discovery Index
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Title: DC.4+.SLN#3
identifier: GSM538247
description:
DC.4+.SLN#3, skin draining LN CD4+dendritic cell
aggregation:
instance of dataset
dateReleased:
04-29-2010
tissue:
Subcutaneous LNs
availability:
available
relatedIdentifiers:
GPL6246
isAbout:
PI SA-PECy7
C57BL/6J
CD11b-FITC CD4-PE CD11c-Alexa750 CD8a-PacificBlue CD45-APC
authorizations:
registration not required
landingPage: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM538247
subject gender: male
subject species: Mus musculus
subject age: 6 weeks
fullName:
Liang Yang
affiliations:
Harvard Medical School
roles:
principal investigator
method:
dissection/trimming
Blendzyme liberase III + DNase digestion CD3, CD19, NK1.1, GR1, Ter119 MACS depletion
instrument:
Affymetrix GeneChip Scanner
information:
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
description:
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
identifier:
GSE15907

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