Immunological Data Discovery Index
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Title: NKT.4-.Sp#2
identifier: GSM538330
description:
NKT.4-.Sp#2, CD4- iNKT cells from Spleen
aggregation:
instance of dataset
dateReleased:
04-29-2010
tissue:
spleen
availability:
available
relatedIdentifiers:
GPL6246
isAbout:
PI CD19 B220 CD11b Ter119 CD4
C57BL/6J
TCRbeta+ aGalCer tet+ CD4-
authorizations:
registration not required
landingPage: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM538330
subject gender: male
subject species: Mus musculus
subject age: 6 weeks
fullName:
Liang Yang
affiliations:
Harvard Medical School
roles:
principal investigator
method:
dissection/trimming
homogenize, ACK lyse, stain MACS enrichment, double sort (FACS Aria)
instrument:
Affymetrix GeneChip Scanner
information:
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
description:
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
identifier:
GSE15907

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