Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SDY225 |
| description: |
A mouse model has been extensively used to investigate disease intervention approaches and correlates of immunity following influenza virus infection. IN and aerosol routes of inoculation were compared and end-points of immunity and disease pathogenesis were evaluated in mice using mouse-mouseadapted H3N2 A/Aichi/2/68 (x31).
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| aggregation: |
instance of dataset
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| refinement: |
2 - Complete set of descriptive data and results, as ascertained by ImmPort.
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| availability: |
available with registration
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| primaryPublications: |
21449723 |
| isAbout: |
Results show that aerosol inoculation with x31 caused more severe lung pathology and enhanced morbidity and mortality compared to IN inoculation with x31. Also, enhanced disease may be associated with increased pulmonary IL-6 expression.
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| authorizations: |
registration required
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| accessURL: |
https://aspera-immport.niaid.nih.gov:9443/browser?path=SDY225 |
| landingPage: |
http://www.immport.org/immport-open/public/study/study/displayStudyDetail/SDY225 |
| clinical trial: | |
| study category: | Vaccine Response |
| study type: | Interventional |
| subject species: | Mus musculus |
| biosample type: |
Other Tissue |
| subject gender: | Female |
| assay type: |
Flow Cytometry Luminex xMAP Microscopy Other |
| name: |
Intranazal and aerosol routes of inoculation were compared and end-points of immunity and disease pathogenesis were evaluated in mice using mouse-adapted H3N2 A/Aichi/2/68 (x31)
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| fullName: |
Ralph Tripp
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| affiliations: |
College of Veterinary Medicine, University of Georgia
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| roles: |
principal investigator
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| name: |
Aerosol Inoculation with a Sub-lethal Influenza Virus Leads to Exacerbated Morbidity and Pulmonary Disease Pathogenesis
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| size: |
5
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| name: |
Influenza Pathogenesis & Immunology Research Center (IPIRC)
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| output: |
HIA, serum microneutralization assay, plaque assay in lung tissue
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| studyGroups: |
X31 intranasal (IN) virus inoculation: mice (5 mice/group) were inoculated by IN instillation of 0.05mL/nare, for a total inoculation of 0.1mL with 106 TCID50/mL x31 virus
X31 Aerosal virus inoculation: mice (5 mice/group) were inoculated for 45 sec using a nebulizer for approximately 750 mL of virus suspension, based on exposure time at a flow rate of 1 cc/min.
PR8 intranasal (IN) virus inoculation: mice were inoculated by IN instillation of PR8
X31-Aerosal with purified rat IgG: mice (9 mice/group) were IP injected with 0.5 mg of a purified rat IgG isotype as control
X31-Aerosal with anti-IL-6 antibody treatment: mice (9 mice/group) were inoculated with X31-Aerosal with anti-IL-6 antibody prophylactic treatment
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| description: |
Examined how the method of inoculation affected immunity and disease pathogenesis in mice using mouseadaptedH3N2 A/Aichi/2/68 (x31)
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| identifier: |
10.21430/M3SE0EVY36
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| name: |
ImmPort
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| identifier: |
SCR:012804
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| homePage: |
http://www.immport.org |
