Reactive oxygen species and TH2 responses to papain | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Reactive oxygen species and TH2 responses to papain

Dataset

identifier:	SDY259
description:	The role of reactive oxygen species in the TH2 response to papain was investigated. The production of ROS by dendritic cells both in vivo and in vitro was examined as an inducer of TH2 differentiation and suppressor of TH1 differentiation. CD70 and IL-12 as effectors of ROS-mediated suppression of TH1 differentiation were investigated as well as the effect of ROS on IL-4-mediated TH2 response.
aggregation:	instance of dataset
refinement:	2 - Complete set of descriptive data and results, as ascertained by ImmPort.
availability:	available with registration
primaryPublications:	20495560
isAbout:	To investigate the mediators of TH2 activation and TH1 suppression in response to papain.

Access

authorizations:	registration required
accessURL:	http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE21602 http://www.bu.edu/computationalimmunology/ https://aspera-immport.niaid.nih.gov:9443/browser?path=SDY259
landingPage:	http://www.immport.org/immport-open/public/study/study/displayStudyDetail/SDY259

Dimension

clinical trial:
study category:	Immune Response
study type:	Interventional
subject species:	Mus musculus
biosample type:	Cell Organ RNA
subject gender:	Unknown
assay type:	DNA microarray Flow Cytometry

Disease

name:	TH2 cell response to challenge with OVA and papain

Person

fullName:	Thomas Kepler
affiliations:	Duke University
roles:	principal investigator

Study Group

name:	Reactive oxygen species and TH2 responses to papain
size:	212

Study

name:	Multiscale Systems Immunology for Adjuvant Development
output:	Flow Cytometry, Gene Expression Microarray
studyGroups:	Duke_Emory_Exp15_BROMELAIN: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+bromelain 50ug Duke_Emory_Exp15_BROMELAIN-NAC: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) OVA 100ug+bromelain 50ug, 2) once daily with NAC (150 mg per kg body weight) from 2 d before to 3 d after immunization Duke_Emory_Exp15_Derf1: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+Derf1 50ug Duke_Emory_Exp15_Derf1-NAC: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+Derf1 50ug, once daily with NAC (150 mg per kg body weight) from 2 d before to 3 d after immunization Duke_Emory_Exp15_Derf1-PBS: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+Derf1 50ug, PBS Duke_Emory_Exp15_Derp1: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+Derp1 50ug Duke_Emory_Exp15_Derp1-NAC: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) OVA 100ug+Derf1 50ug, 2) once daily with NAC (150 mg per kg body weight) from 2 d before to 3 d after immunization Duke_Emory_Exp15_Derp1-PBS: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+Derp1 50ug, PBS Duke_Emory_Exp15_PBS: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp15_SEA: Mouse strain: 4get mouse, BALB/c background, Subject treatment: OVA 100ug+SEA 50ug Duke_Emory_Exp15_SEA-NAC: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) OVA 100ug+SEA 50ug, 2) once daily with NAC (150 mg per kg body weight) from 2 d before to 3 d after immunization Duke_Emory_Exp16_WT_naive: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive Duke_Emory_Exp18_naive: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive Duke_Emory_Exp18_Nrf2 ko_naive: Mouse strain: Nrf2 ko, Subject treatment: naive Duke_Emory_Exp18_P47 ko_naive: Mouse strain: P47 KO, Subject treatment: naive Duke_Emory_Exp18_TLR4ko_naive: Mouse strain: TLR4 KO, Subject treatment: naive Duke_Emory_Exp18_WT_naive: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive Duke_Emory_Exp18_WT_papain: Mouse strain: C57BL/6 wild type mice, Subject treatment: papain 50ug Duke_Emory_Exp20_IFN-a ko: Mouse strain: IFN-alpha KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp20_JNK1 ko: Mouse strain: JNK1 KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp20_JNK1 ko NACs: Mouse strain: JNK1 KO, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp20_PAR1 ko: Mouse strain: PAR1 KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp20_PAR1 ko NACS: Mouse strain: PAR1 KO, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp20_WT: Mouse strain: C57BL/6 wild type mice, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp20_wt for IFN-a: Mouse strain: wt control mice for IFN-alpha KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp20_WT NACs: Mouse strain: C57BL/6 wild type mice, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp24_ERK ko: Mouse strain: ERK KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp24_ERK ko NACs: Mouse strain: ERK KO, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp24_JNK1 ko: Mouse strain: JNK1 KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp24_JNK1 ko-NACs: Mouse strain: JNK1 KO, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp24_NOX2 ko: Mouse strain: NOX2 KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp24_NOX2 ko-NACs: Mouse strain: NOX2 KO, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp24_TLR6 ko: Mouse strain: TLR6 KO, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp24_TLR6 ko-NACs: Mouse strain: TLR6 KO, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp24_WT: Mouse strain: C57BL/6 wild type mice, Subject treatment: OVA 100ug+papain 50ug Duke_Emory_Exp24_WT_NAC: Mouse strain: C57BL/6 wild type mice, Subject treatment: OVA 100ug+papain 50ug, NAC 150mg/kg body weight Duke_Emory_Exp25_hirudinMMP1: Mouse strain: 4get, Subject treatment: 1) papain 50ug 2) hirudin 120 mg/kg body weight Duke_Emory_Exp25_lepirudin: Mouse strain: 4get, Subject treatment: 1) papain 50ug 2) lepirudin 1 mg/kg body weight Duke_Emory_Exp25_PBS: Mouse strain: 4get, Subject treatment: 1) papain 50ug 2) PBS Duke_Emory_Exp26_B6: Mouse strain: C57BL/6 wild type mice, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_B6-NACs: Mouse strain: C57BL/6 wild type mice, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_MyD88-LPS: Mouse strain: MyD88 ko, Subject treatment: 1) OVA 100ug+LPS 50ug 2) PBS Duke_Emory_Exp26_MyD88-LPS-NAC: Mouse strain: MyD88 ko, Subject treatment: 1) OVA 100ug+LPS 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_MyD88-NACs-papain: Mouse strain: MyD88 ko, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_MyD88-papain: Mouse strain: MyD88 ko, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_NAC_4get: Mouse strain: 4get, Subject treatment: 1) OVA 100ug+papain 50ug 2)once daily with NAC (150 mg per kg body weight) from 2 d before to 3 d after immunization Duke_Emory_Exp26_NACs_WT: Mouse strain: C57BL/6 wild type mice, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_no NAC_4get: Mouse strain: 4get, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_no NACs_WT: Mouse strain: C57BL/6 wild type mice, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_PBS: Mouse strain: 4get, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_TLR2: Mouse strain: TLR2 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_TLR2-NACs: Mouse strain: TLR2 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_TLR3: Mouse strain: TLR3 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_TLR3-NACs: Mouse strain: TLR3 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_TLR6: Mouse strain: TLR6 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_TLR6-NACs: Mouse strain: TLR6 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_TLR7: Mouse strain: TLR7 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_TLR7-NACs: Mouse strain: TLR7 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_TLR9: Mouse strain: TLR9 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) PBS Duke_Emory_Exp26_TLR9-NACs: Mouse strain: TLR9 KO, Subject treatment: 1) OVA 100ug+papain 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp26_WT-LPS: Mouse strain: C57BL/6 wild type mice, Subject treatment: 1) OVA 100ug+LPS 50ug 2) PBS Duke_Emory_Exp26_WT-LPS-NAC: Mouse strain: C57BL/6 wild type mice, Subject treatment: 1) OVA 100ug+LPS 50ug 2) NAC 150mg/kg body weight Duke_Emory_Exp27_blank paricles 6: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) blank poly microparticles 6ug 2) OVA 100ug+papain 50ug Duke_Emory_Exp27_blank particles 0.67: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) blank poly microparticles 0.67ug 2) OVA 100ug+papain 50ug Duke_Emory_Exp27_blank particles2: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) blank poly microparticles 2ug 2) OVA 100ug+papain 50ug Duke_Emory_Exp27_TEMPOL PARTICLES 0.67: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1) TEMPOL PARTICLES 0.67ug on day -1 2) OVA 100ug+papain 50ug Duke_Emory_Exp27_TEMPOL PARTICLES 2: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1)TEMPOL PARTICLES 2ug on day -1 2) OVA 100ug+papain 50ug Duke_Emory_Exp27_TEMPOL PARTICLES 6: Mouse strain: 4get mouse, BALB/c background, Subject treatment: 1)TEMPOL PARTICLES 6ug on day -1 2) OVA 100ug+papain 50ug Duke_Emory_Exp29_WT_naive: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive Duke_Emory_Exp33_WT_naive: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive Duke_Emory_Exp43_WT_naive: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive Duke_Emory_Microarray_WT: Mouse strain: C57BL/6 wild type mice, Subject treatment: naive
description:	The role of reactive oxygen species in the TH2 response to papain was investigated. The production of ROS by dendritic cells both in vivo and in vitro was examined as an inducer of TH2 differentiation and suppressor of TH1 differentiation. CD70 and IL-12 as effectors of ROS-mediated suppression of TH1 differentiation were investigated as well as the effect of ROS on IL-4-mediated TH2 response.
identifier:	10.21430/M3DWBGXETN

Data Repository

name:	ImmPort
identifier:	SCR:012804
homePage:	http://www.immport.org

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