Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SDY22 |
| description: |
The objective is to understand role of polymorphisms in genes of innate and adaptive immunity in modulating the response to vaccines for typhoid gastrointestinal tract infections by:
Collecting blood and saliva from 1000 vaccine study participants for each vaccine in Kolkata, India, at days 1, 3, and 28.
Determining vaccine-specific titers using ELISA, vibriocidal, and multiplex bead antibody assays.
Discover and validate SNP polymorphisms in this population and screen all participant's DNA for SNPs to 14 innate immunity genes and genome scans for adaptive immunity genes.
Characterize protein/peptide expression using LC/MS, immunoassay, and flow cytometry for protein mediators of innate/adaptive immunity and 22 proteins involved in cell stimulation and signal transduction; and 2-D gel MS/MS on 100-200 proteins, protein isoforms and protein fragments to determine more generalized effects of immune activation, signal transduction and general response to vaccination.
Determine haplotypes, perform association analyses between vaccine response (defined either categorically or quantitatively) and SNPs, haplotypes, or protein expression parameters, and perform association analyses between genomic and proteomic assessments.
Conduct resequencing of genes with SNPs that are significantly associated to vaccine response.
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| aggregation: |
instance of dataset
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| refinement: |
2 - Complete set of descriptive data and results, as ascertained by ImmPort.
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| availability: |
available with registration
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| primaryPublications: |
20107010 23583812 |
| isAbout: |
The objective is to understand role of polymorphisms in genes of innate and adaptive immunity in modulating the response to typhoid vaccine.
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| authorizations: |
registration required
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| accessURL: |
https://aspera-immport.niaid.nih.gov:9443/browser?path=SDY22 |
| landingPage: |
http://www.immport.org/immport-open/public/study/study/displayStudyDetail/SDY22 |
| clinical trial: | |
| study category: | Vaccine Response |
| study type: | Interventional |
| subject species: | Homo sapiens |
| biosample type: |
DNA Saliva Serum |
| subject gender: | Both |
| assay type: |
ELISA Luminex xMAP Mass Spectrometry Other SNP microarray Sequencing |
| name: |
Responses to typhoid vaccines by a population stratified by age, gender and ethnicity.
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| fullName: |
Diane Wagener
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| affiliations: |
Research Triangle Institute, NC
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| roles: |
principal investigator
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| name: |
Genotype Polymorphism effects on Typhoid Vaccine responses.
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| size: |
1003
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| name: |
Immune Response Polymorphisms: Typhoid/Cholera Vaccines
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| output: |
Standard ELISA assays for measuring anti-Vi IgM, IgA and IgG titers.
Widal, Tubex and Typhidot-M tests for determination of typhoid in the event of serious adverse reactions following vaccination.
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| studyGroups: |
Typhoid Vaccine Recipients: 1,000 participants receiving Salmonella typhi vaccine studied in a stratified random sampling design for gender, age and ethnicity.
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| description: |
Innate immunity gene products are known to be the first-line of host-defense and to control and assist the adaptive immune system. This research represents the opportunity to study, in detail, the responses longitudinally to vaccination (using clinical examination and laboratory assays) in a large ethnic population. Consequently, a prioritized set of genes and gene products from both systems will be evaluated.
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| identifier: |
10.21430/M36AEP1T4U
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| name: |
ImmPort
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| identifier: |
SCR:012804
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| homePage: |
http://www.immport.org |
