Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SDY110 |
| description: |
We have developed for the first time a pig model to study mucosal and systemic Th1 and CD8+ cytotoxic immune responses to H. pylori infection.
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| aggregation: |
instance of dataset
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| refinement: |
2 - Complete set of descriptive data and results, as ascertained by ImmPort.
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| availability: |
available with registration
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| primaryPublications: |
23897614 |
| isAbout: |
Based on our hypothesis the aim of this project was to overcome the limitation in the study of CD8+ T cell responses to H. pylori.
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| authorizations: |
registration required
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| accessURL: |
http://modelingimmunity.vbi.vt.edu/ https://aspera-immport.niaid.nih.gov:9443/browser?path=SDY110 |
| landingPage: |
http://www.immport.org/immport-open/public/study/study/displayStudyDetail/SDY110 |
| clinical trial: | |
| study category: | Infection Response |
| study type: | Longitudinal |
| subject species: | Sus scrofa domesticus |
| biosample type: |
Cell Other Protein RNA Tissue |
| subject gender: | Both |
| assay type: |
Flow Cytometry Other Q-PCR |
| name: |
Pigs were divided into 3 groups of uninfected, infected with H. pylori strain J99 and infected with H. pylori strain SS1.
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| fullName: |
Josep Bassaganya-Riera
Raquel Hontecillas
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| affiliations: |
NIMML
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| roles: |
principal investigator
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| name: |
Immune responses to Helicobacter pylori in a pig model
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| size: |
15
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| name: |
Virginia Bioinformatics Institute Modeling Immunity for Biodefense Contract
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| output: |
Bacterial challenge with H. pylori, assessment of systemic immune responses over time and local immune responses as well as pathology.
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| studyGroups: |
Case: C. difficille infected
Control: Control group
Unknown: Unknown
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| description: |
Helicobacter pylori infection is the leading cause for peptic ulcer disease and gastric adenocarcinoma. Mucosal T cell responses play an important role in mediating H. pylori-related gastric immunopathology. While induced regulatory T (iTreg) cells are required for chronic colonization without disease, Th1 effector responses are associated with lower bacterial load at the expense of gastric pathology. With the objective of developing a large animal model of H. pylori infection, pigs were inoculated with either H. pylori strain SS1 or J99. Changes in peripheral blood mononuclear cell populations were monitored weekly, and mucosal immune responses and bacterial loads were assessed at day 57 post-infection. Both H. pylori strains elicited a Th1 response with increased percentage of CD4+Tbet+ cells and elevated Tbet and IFN-. mRNA in PBMC. A subset of CD8beta+ T cells and B cells expressing Tbet increased due to infection. Moreover, a significant increase of NK cells was observed in infected pigs pointing towards a predominant cytotoxic immune response. Infiltration of cytotoxic cells was detected in the gastric lamina propria of both infected groups. Interestingly, strain J99 evoked a more dramatic acute response associated with lower bacterial loads, while strain SS1 showed longer-term colonization capacity. This novel pig model of infection closely mimics human gastric pathology and presents a suitable avenue for studying the cytotoxic and regulatory responses towards H. pylori described in humans.
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| identifier: |
10.21430/M3WXZHC81N
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| name: |
ImmPort
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| identifier: |
SCR:012804
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| homePage: |
http://www.immport.org |
