Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SDY572 |
| description: |
Enteroaggregative Escherichia coli (EAEC) is increasingly recognized as a major cause of diarrheal disease globally. In the current study, we investigated the impact of zinc deficiency on the host and pathogenesis of EAEC. Several outcomes of EAEC infection were investigated including weight loss, EAEC shedding and tissue burden, leukocyte recruitment, intestinal cytokine expression, and virulence expression of the pathogen in vivo. Mice fed a protein source defined zinc deficient diet (dZD) had an 80% reduction of serum zinc and a 50% reduction of zinc in luminal contents of the bowel compared to mice fed a protein source defined control diet (dC). When challenged with EAEC, dZD mice had significantly greater weight loss, stool shedding, mucus production, and, most notably, diarrhea compared to dC mice. Zinc deficient mice had reduced infiltration of leukocytes into the ileum in response to infection suggesting an impaired immune response. Interestingly, expression of several EAEC virulence factors were increased in luminal contents of dZD mice. These data show a dual effect of dietary zinc in benefitting the host while impairing virulence of the pathogen. The study demonstrates the critical importance of zinc and may help elucidate the benefits of zinc supplementation in cases of childhood diarrhea and malnutrition.
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| aggregation: |
instance of dataset
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| refinement: |
2 - Complete set of descriptive data and results, as ascertained by ImmPort.
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| availability: |
available with registration
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| primaryPublications: |
25483331 |
| isAbout: |
The goal of this study is isolate the variable of zinc deficiency in the control and malnourished mice to determine their importance to the model.
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| authorizations: |
registration required
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| accessURL: |
http://www.modelingimmunity.org/ https://aspera-immport.niaid.nih.gov:9443/browser?path=SDY572 |
| landingPage: |
http://www.immport.org/immport-open/public/study/study/displayStudyDetail/SDY572 |
| clinical trial: | |
| study category: |
Immune Response Infection Response |
| study type: | Longitudinal |
| subject species: | Mus musculus |
| biosample type: |
DNA Other RNA Tissue |
| subject gender: | Male |
| assay type: |
ELISA Mass Spectrometry Other Q-PCR |
| name: |
Host responses to EAEC infection and modulation by nutrition.
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| fullName: |
Raquel Hontecillas
Richard Guerrant
Josep Bassaganya-Riera
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| affiliations: |
NIMML
UVA
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| roles: |
principal investigator
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| name: |
Host responses to Enteroaggregative Escherichia coli (EAEC) infection
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| size: |
159
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| name: |
Virginia Bioinformatics Institute Modeling Immunity for Biodefense Contract
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| output: |
Tissue burden, weights, bacterial shedding in stool, zinc levels in serum, tissue, qPCR
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| studyGroups: |
EAEC40 Infected: EAEC 042 Infected
EAEC40 Uninfected: Uninfected
Unknown: Unknown
EAEC51 Infected: EAEC 042 Infected
EAEC51 Uninfected: Uninfected
EAEC52 Infected: EAEC infected
EAEC52 Uninfected: Uninfected
EAEC53 Infected: EAEC infected
EAEC53 Uninfected: Uninfected
AIN93G Nourished Diet: Control
Nourished AA-defined: Control
Tryptophan Deficient AA-defined: Control
No tryptophan supplementation: Case
1mg/d tryptophan supplementation: Case
5mg/d tryptophan supplementation: Case
15mg/d tryptophan supplementation: Case
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| description: |
Enteroaggregative Escherichia coli (EAEC) is increasingly recognized as a major cause of diarrheal disease globally. In the current study, we investigated the impact of zinc deficiency on the host and pathogenesis of EAEC. Mouse model of enteroaggregative E. coli infection in mice that are fed one of four different diets: Control diet (20% protein) Control diet - Zn (20% protein, no zinc) RBD (7% protein) RBD/ZD (7% protein, no zinc)
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| identifier: |
10.21430/M3G6QJN9D5
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| startDate: |
2013-05-28
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| name: |
ImmPort
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| identifier: |
SCR:012804
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| homePage: |
http://www.immport.org |
