Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SRR1976591 |
| cellType: |
CD4 TConv Cell
|
| alternateIdentifiers: |
SRS911758
|
| description: |
Total CD4 (TCRB+CD4+)
|
| aggregation: |
instance of dataset
|
| dateReleased: |
04-21-2015
|
| tissue: |
Spleen
|
| availability: |
available
|
| relatedIdentifiers: |
SAMN03487352
|
| isAbout: |
CD4+TCRB+
C57BL/6J
|
| authorizations: |
registration not required
|
| accessURL: |
https://www.ncbi.nlm.nih.gov/biosample/SAMN03487352 |
| landingPage: |
https://www.ncbi.nlm.nih.gov/sra/SRX997539 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 5 week-old |
| affiliations: |
Harvard Medical School
|
| fullName: |
Christophe Benoist
|
| roles: |
principal investigator
|
| instrument: |
Illumina HiSeq 2500
|
| information: |
TRANSCRIPTOMIC
PolyA
PAIRED
|
| method: |
FACSAria
RNA-Seq
|
| platform: |
Model organism or animal; version 1.0
|
| name: |
Spleen CD4 T Cell from Mus musculus
|
| identifier: |
SRX997539
|
| description: |
RNA was prepared from 100,000 cells (final sort collected directly in Trizol). Total RNA was extracted from each sample following ImmGen RNA extraction SOP. PolyA+ RNA was isolated by bead capture (PrepX PolyA mRNA Isolation Kit, Wafergen Biosystems), following the manufacturer’s instructions. Then mRNA libraries were prepared with the PrepX RNA-Seq Library Preparation Kit (Integenex). For first strand synthesis, mRNA was fragmented and ligated to 3’ and 5’ adapters before reverse transcription. Sample libraries then underwent bead clean-up, 2nd strand synthesis and PCR amplification before final sequencing. Libraries were analyzed on an Illumina HiSeq2500 sequencer.
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |
