Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.

instance of dataset

available with registration

NIAID (HIPC funded)

SDY212

SDY887

1. Compare lymphocyte responses at Days 5-7 and the lymphocyte and serology responses at Day 28 post-immunization following administration of seasonal trivalent inactivated influenza vaccine. 2. Evaluate changes in cytokine profile in the immune response (CD4, CD8, gamma delta cells, B cell responses) from Day 0 to Day 5-7 for T cells and antibody-secreting cells (ASCs). 3. Evaluate changes in cytokine profile in the immune response (CD4, CD8, gamma delta cells, B cell, and serology responses) from Day 0 to Day 28 for HAI responses. 4. Evaluate any potential gene expression signatures either at Day 0 or during the early response period Day 5-7 that are associated with immune senescence, potentially providing insight into mechanism. 5. Compare monocyte reactivity to activating stimuli at Days 0 and 5-7 with the lymphocyte and serology responses at Day 28 post-immunization following administration of seasonal trivalent inactivated influenza vaccine (Year 1 only).

registration required

Flow cytometry analyzed results

Hemagglutination inhibition (HAI)

Multiplex bead array asssay

FCS control files

FCS sample files

Gene expression microarray data files

Aging, Immune responses to influenza vaccine.

Stanford University

Mark Davis

principal investigator

T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2009 (See companion studies SDY315 2012 / SDY314 2008 / SDY311 2010 / SDY112 2011)

84

1. Combinatorial tetramer analysis by FACS and/or CyTOF; Phosphoflow. 2. Luminex; Gene expression profiling (Affymetrix microarray). 3. Luminex; Gene expression profiling (Affymetrix microarry); Microneutralization assays. 4. Gene expression profiling (Affymetrix microarray); Immune phenotyping by CyTOF. 5. Gene expression profiling (Affymetrix microarray). 6. Immune phenotyping by CyTOF; Luminex. 7. ELISA

Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.

ImmuneSpace