Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SDY315 |
| description: |
Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
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| aggregation: |
instance of dataset
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| availability: |
available with registration
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| acknowledges: |
NIAID (HIPC funded)
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| isAbout: |
1. Compare lymphocyte responses at Days 6-8 and the lymphocyte and serology responses at Day 28 post-immunization following administration of seasonal trivalent inactivated influenza vaccine. 2. Evaluate changes in cytokine profile in the immune response (CD4, CD8, gamma-delta cells, B cell responses) from Day 0 to Day 6-8 for T cells and antibody-secreting cells (ASCs). 3. Evaluate changes in cytokine profile in the immune response (CD4, CD8, gamma-delta cells, B cell, and serology responses) from Day 0 to Day 28 for HAI responses. 4. Evaluate any potential gene expression signatures either at Day 0 or during the early response period Day 6-8 that are associated with immune senescence, potentially providing insight into mechanism. 5. Compare cell specific gene expression differences between age groups for both whole blood and whole PBMC. 6. Determine the effect of cell subset frequency variation on serum cytokine abundance. 7. Evaluate CMV and EBV seroreactivity at Day 0 and additional timepoints throughout the study as able.
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| authorizations: |
registration required
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| types: |
Flow cytometry analyzed results
Hemagglutination inhibition (HAI)
Multiplex bead array asssay
FCS control files
FCS sample files
Gene expression microarray data files
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| accessURL: |
https://www.immunespace.org/study/Studies/SDY315/dataset.view?datasetId=5017 https://www.immunespace.org/study/Studies/SDY315/dataset.view?datasetId=5005 https://www.immunespace.org/study/Studies/SDY315/dataset.view?datasetId=5008 https://www.immunespace.org/study/Studies/SDY315/dataset.view?datasetId=5019 https://www.immunespace.org/study/Studies/SDY315/dataset.view?datasetId=5018 https://www.immunespace.org/study/Studies/SDY315/dataset.view?datasetId=5014 |
| landingPage: |
https://www.immunespace.org/project/Studies/SDY315/begin.view? |
| subject species: | Homo sapiens |
| study conditions: | Influenza |
| study type: | Longitudinal |
| study category: | Vaccine Response |
| assay type: |
CyTOF Flow Cytometry Gene Expression HAI MBAA |
| time point: | Unknown |
| subject race: |
American Indian or Alaska Native Asian Other Unknown White |
| subject gender: |
Female Male |
| subject age: |
21-30 31-40 51-60 61-70 > 70 |
| name: |
Aging, Immune responses to influenza vaccine.
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| affiliations: |
Stanford University
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| fullName: |
Mark Davis
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| roles: |
principal investigator
|
| name: |
T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2012 (See companion studies SDY311 2010 / SDY312 2009 / SDY314 2008 / SDY112 2011)
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| size: |
74
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| output: |
1. Combinatorial tetramer analysis by FACS and/or CyTOF; Phosphoflow. 2. Luminex; Gene expression profiling (Affymetrix microarray). 3. Luminex; Gene expression profiling (Affymetrix microarry); Microneutralization assays. 4. Gene expression profiling (Affymetrix microarray); Immune phenotyping by CyTOF. 5. Gene expression profiling (Affymetrix microarray). 6. Immune phenotyping by CyTOF; Luminex. 7. ELISA
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| description: |
Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
|
| name: |
ImmuneSpace
|
| homePage: |
http://www.immunespace.org |
