Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | SDY28 |
| description: |
Our broad objective is to examine the role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia vaccine-induced humoral and cell-mediated immune responses among a cohort of 1,000 recently vaccinated subjects.In Research Area 1, we propose to study associations between specific class I and II HLA alleles (HLA-A, -B, -C, -DRB, -DQA, -DQB, -DPA, -DPB), specific cytokine genes, polymorphisms of the above cytokine receptors, a genome-wide SNP analysis; and variations in immune response to smallpox vaccine.In Research Area 2, we focus on identifying associations between expression and function of these same candidate genes likely to regulate immune response variations and humoral and cell-mediated immune responses following smallpox vaccination in selected human subjects. Both gene products (i.e., secreted proteins), cell surface expression, and measures of gene regulation/activation will be pursued. We focus on gene families involved in initiating, sustaining and regulating innate and adaptive immune responses, as well as those directly involved in directing specific antibody and cytotoxic T cell responses.
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| aggregation: |
instance of dataset
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| availability: |
available with registration
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| acknowledges: |
NIAID
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| primaryPublications: |
23594957 23806267 27513748 22949304 19038260 |
| isAbout: |
To correlate variability in humoral and cell-mediated immune responses to vaccinia virus immunization with genetic variation in the HLA, cytokine and cytokine receptor genes and the broader genome.
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| authorizations: |
registration required
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| types: |
Enzyme-linked immunosorbent assay (ELISA)
Flow cytometry analyzed results
Human leukocyte antigen (HLA) typing
FCS sample files
Gene expression microarray data files
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| accessURL: |
https://www.immunespace.org/study/Studies/SDY28/dataset.view?datasetId=5003 https://www.immunespace.org/study/Studies/SDY28/dataset.view?datasetId=5017 https://www.immunespace.org/study/Studies/SDY28/dataset.view?datasetId=5006 https://www.immunespace.org/study/Studies/SDY28/dataset.view?datasetId=5018 https://www.immunespace.org/study/Studies/SDY28/dataset.view?datasetId=5014 |
| landingPage: |
https://www.immunespace.org/project/Studies/SDY28/begin.view? |
| subject species: | Homo sapiens |
| study conditions: | Vaccinia |
| study type: | Observational |
| study category: | Vaccine Response |
| assay type: |
ELISA Flow Cytometry Gene Expression HLA Typing |
| time point: | |
| subject race: |
American Indian or Alaska Native Black or African American Other Unknown White |
| subject gender: |
Female Male Not Specified |
| subject age: |
11-20 21-30 31-40 41-50 Unknown |
| name: |
Role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia-induced humoral and cell-mediated immune responses among a cohort of 1,000 recently vaccinated subjects.
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| affiliations: |
Mayo Vaccine Research Group
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| fullName: |
Gregory Poland
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| roles: |
principal investigator
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| name: |
Humoral and Cell-Mediated Immune Responses to Vaccinia Virus Immunization
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| size: |
1092
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| output: |
ELISA-neutralizing antibody titers.ELISPOT-Antigen specific cells.HLA Genotyping- A, B, C, DRB, DQA, DPA, DPB.SNP Genotyping of Cytokine and cytokine receptor genes.Genome Wide Association Study (GWAS).Microarray analysis of PBMC responses.ELISA- Cytokines.Flow Cytometry-HLA Class I & II.
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| description: |
Our broad objective is to examine the role of candidate human immune response gene polymorphisms (and their receptors, expression and function) in inter-individual variability in vaccinia vaccine-induced humoral and cell-mediated immune responses among a cohort of 1,000 vaccinated subjects.
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| name: |
ImmuneSpace
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| homePage: |
http://www.immunespace.org |
