Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | SC.STSL.BM#3 |
| identifier: | GSM399455 |
| description: |
SC.STSL.BM#3, short-term reconstituting stem cell
|
| aggregation: |
instance of dataset
|
| dateReleased: |
05-02-2009
|
| tissue: |
Bone marrow
|
| availability: |
available
|
| relatedIdentifiers: |
GPL6246
|
| isAbout: |
Ly6c Ter119 CD11b CD3 GR1 IgM CD24
C57BL/6J
CD117+ IL7R- CD150- CD48- AA4.1+ CD43+
|
| authorizations: |
registration not required
|
| landingPage: |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM399455 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 11 weeks |
| fullName: |
Liang Yang
|
| affiliations: |
Harvard Medical School
|
| roles: |
principal investigator
|
| method: |
dissection/trimming
disruption by passing through 25g needle/1ml syringe, ACK lysis of RBCs 1x flow sorting
|
| instrument: |
Affymetrix GeneChip Scanner
|
| information: |
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
|
| description: |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| identifier: |
GSE15907
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |