Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | DC.8+.MLN#1 |
| identifier: | GSM538252 |
| description: |
DC.8+.MLN#1, mesenteric LN CD8+ dendritic cell
|
| aggregation: |
instance of dataset
|
| dateReleased: |
04-29-2010
|
| tissue: |
Mesenteric LN
|
| availability: |
available
|
| relatedIdentifiers: |
GPL6246
|
| isAbout: |
PI SA-PECy7
C57BL/6J
CD11b-FITC CD4-PE CD11c-Alexa750 CD8a-PacificBlue CD45-APC
|
| authorizations: |
registration not required
|
| landingPage: |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM538252 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 6 weeks |
| fullName: |
Liang Yang
|
| affiliations: |
Harvard Medical School
|
| roles: |
principal investigator
|
| method: |
dissection/trimming
Blendzyme liberase III + DNase digestion CD3, CD19 MACS depletion
|
| instrument: |
Affymetrix GeneChip Scanner
|
| information: |
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
|
| description: |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| identifier: |
GSE15907
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |