Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | NKT.4+.Sp#3 |
| identifier: | GSM538324 |
| description: |
NKT.4+.Sp#3, CD4+ iNKT cells from Spleen
|
| aggregation: |
instance of dataset
|
| dateReleased: |
04-29-2010
|
| tissue: |
spleen
|
| availability: |
available
|
| relatedIdentifiers: |
GPL6246
|
| isAbout: |
CD19 B220 CD11b Ter119 PI
C57BL/6J
TCRb aGalCer-loaded CD1d-tetramer CD4
|
| authorizations: |
registration not required
|
| landingPage: |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM538324 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 6 weeks |
| fullName: |
Liang Yang
|
| affiliations: |
Harvard Medical School
|
| roles: |
principal investigator
|
| method: |
dissection/trimming
homogenized, ACK lyszed, stained MACS enrichement, double sort on FACS Aria
|
| instrument: |
Affymetrix GeneChip Scanner
|
| information: |
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
|
| description: |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| identifier: |
GSE15907
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |