Immunological Data Discovery Index
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Title: T.4Mem.LN#1
identifier: GSM538362
description:
T.4Mem.LN#1, Memory T4 from LNs
aggregation:
instance of dataset
dateReleased:
04-29-2010
tissue:
Subcutaneous LNs
availability:
available
relatedIdentifiers:
GPL6246
isAbout:
PI CD8 CD11b CD11c CD19 CD25 CD49b Gr-1 Ter119
C57BL/6J
TCRb CD44high CD122lo CD4
authorizations:
registration not required
landingPage: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM538362
subject gender: male
subject species: Mus musculus
subject age: 6 weeks
fullName:
Liang Yang
affiliations:
Harvard Medical School
roles:
principal investigator
method:
dissection/trimming
Glass slide teasing, ACK lysis Two round sort on ARIA
instrument:
Affymetrix GeneChip Scanner
information:
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
description:
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
identifier:
GSE15907