Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | DC.8+.Th#2 |
| identifier: | GSM605829 |
| description: |
DC.8+.Th#2, thymus CD8+ DCs
|
| aggregation: |
instance of dataset
|
| dateReleased: |
10-08-2010
|
| tissue: |
thymus
|
| availability: |
available
|
| relatedIdentifiers: |
GPL6246
|
| isAbout: |
Streptavidin-PECy7, propidium iodide
C57BL/6J
CD11b-FITC CD4-PE MHCII-APC CD11c-eFluor780 CD8-eFluor450
|
| authorizations: |
registration not required
|
| landingPage: |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM605829 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 6 weeks |
| fullName: |
Liang Yang
|
| affiliations: |
Harvard Medical School
|
| roles: |
principal investigator
|
| method: |
dissection/trimming
gentle teasing using forceps, Liberase DL + DNAse I digestion, red blood cell lysis, depletion of NK1.1, GR1, CD19, CD3e, Ter119 positive cells using MACS system
|
| instrument: |
Affymetrix GeneChip Scanner
|
| information: |
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
|
| description: |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| identifier: |
GSE15907
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |