Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | preT.DN2B.Th#1 |
| identifier: | GSM791141 |
| description: |
preT.DN2B.Th#1, DN2b thymocytes
|
| aggregation: |
instance of dataset
|
| dateReleased: |
09-07-2011
|
| tissue: |
thymus
|
| availability: |
available
|
| relatedIdentifiers: |
GPL6246
|
| isAbout: |
Lin= CD4, CD8a, TCRb, TCRgd, CD11b, CD11c, NK1.1, Gr1, Ter119; additional B cell exclusion markers CD19, CD138, B220, IgL-kappa, IgL-lamda
C57BL/6J
Lin-/lo CD25hi CD44+ cKitint
|
| authorizations: |
registration not required
|
| landingPage: |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM791141 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 6 weeks |
| fullName: |
Liang Yang
|
| affiliations: |
Harvard Medical School
|
| roles: |
principal investigator
|
| method: |
dissection/trimming
Filter/syringe plunger teasing, ACK lysis Lin+ magnetic depletion (Dynobeads), 2 rounds of sorting, 2d sort directly into trizol
|
| instrument: |
Affymetrix GeneChip Scanner
|
| information: |
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
|
| description: |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| identifier: |
GSE15907
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |