Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | DC.pDC.8+.SLN#2 |
| identifier: | GSM854300 |
| description: |
DC.pDC.8+.SLN#2, Skin lymph node CD8+ plasmacytoid dendritic cells CD11cint/CD8a+/CD4+/B220+/GR1+
|
| aggregation: |
instance of dataset
|
| dateReleased: |
12-28-2011
|
| tissue: |
Skin LN
|
| availability: |
available
|
| relatedIdentifiers: |
GPL6246
|
| isAbout: |
PI SA-PECy7
C57BL/6J
B220-FITC CD45-APC CD11c-eFluor780 GR1-PE CD8-eFluor450
|
| authorizations: |
registration not required
|
| landingPage: |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM854300 |
| subject gender: | male |
| subject species: | Mus musculus |
| subject age: | 6 weeks |
| fullName: |
Liang Yang
|
| affiliations: |
Harvard Medical School
|
| roles: |
principal investigator
|
| method: |
dissection/trimming
gentle teasing using forceps, Liberase DL + DNase I digestion 15 min at 37C depletion of NK1.1, CD3e, CD19 and TER119 positive cells using MACS system
|
| instrument: |
Affymetrix GeneChip Scanner
|
| information: |
RNA
total RNA
Biotin
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Affy 1.0 ST Hyb Kit
|
| description: |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| identifier: |
GSE15907
|
| name: |
immgen
|
| homePage: |
http://www.immgen.org/ |