Synthetic peptide vaccine development: measurement of polyclonal antibody affinity and cross-reactivity using a new peptide capture and release system for surface plasmon resonance spectroscopy.

Title:

Synthetic peptide vaccine development: measurement of polyclonal antibody affinity and cross-reactivity using a new peptide capture and release system for surface plasmon resonance spectroscopy.

Dataset

identifier:	1464256
description:	epitope description:KCKSDQDPQFIPKGCSK host organism:Oryctolagus cuniculus antibody name:L169 (PAK*) (anti-T130K/E135P sera)
aggregation:	instance of dataset
availability:	available
primaryPublications:	15386623

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1007066
landingPage:	http://www.iedb.org/assay/1464256

Publication

type:	Literature
publicationVenue:	J Mol Recognit
dates:	2004

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Paul J Cachia Daniel J Kao Robert S Hodges

Data Type

method:	surface plasmon resonance (SPR)

Study

name:	Synthetic peptide vaccine development: measurement of polyclonal antibody affinity and cross-reactivity using a new peptide capture and release system for surface plasmon resonance spectroscopy.
description:	This is a consensus sequence derived from the PAK, PAO, KB7, and P1 pilin epithelial cell-surface binding domains. The antigen carrier is a synthetic peptide construct (E-coil) containing the modified amino acid norleucine (X). The peptide antigen forms a loop via an internal disulfide bridge, and is covalently bound at the amino terminus to the carrier by specific labeling of a free cysteine in the carrier with iodoacetylated peptide. .

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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