Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1007066 |
| landingPage: |
http://www.iedb.org/assay/1464260 |
| type: |
Literature
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| publicationVenue: |
J Mol Recognit
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| dates: |
2004
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| study type: | b cell assays |
| subject species: |
| fullName: |
Paul J Cachia
Daniel J Kao
Robert S Hodges
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| method: |
surface plasmon resonance (SPR)
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| name: |
Synthetic peptide vaccine development: measurement of polyclonal antibody affinity and cross-reactivity using a new peptide capture and release system for surface plasmon resonance spectroscopy.
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| description: |
This is a consensus sequence derived from the PAK, PAO, KB7, and P1 pilin epithelial cell-surface binding domains.
The antigen carrier is a synthetic peptide construct (E-coil) containing the modified amino acid norleucine (X). The peptide antigen forms a loop via an internal disulfide bridge, and is covalently bound at the amino terminus to the carrier by specific labeling of a free cysteine in the carrier with iodoacetylated peptide. Antiserum from a second rabbit responded with a two-fold higher KD to this consensus epitope. One of two rabbits immunized with the tetanus toxoid-conjugated peptide also responded to this consensus epitope with a two-log higher KD. .
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
