Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants.

Dataset

identifier:	1378523
description:	epitope description:(2R,3R,23Z,33Z)-3-hydroxy-2-octyldopentaconta-23,33-dienoic acid antigen name:glucosylceramide host organism:Mus musculus BALB/c antibody name:MEST-2
aggregation:	instance of dataset
availability:	available
primaryPublications:	11287397

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1003832
landingPage:	http://www.iedb.org/assay/1378523

Publication

type:	Literature
publicationVenue:	Glycobiology
dates:	2001

Dimension

study type:	b cell assays
subject species:	Paracoccidioides brasiliensis

Person

fullName:	M S Toledo E Suzuki S B Levery A H Straus H K Takahashi

Data Type

method:	western blot

Study

name:	Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants.
description:	The epitope is derived from glucosylceramide of fungi and plants. The epitope specific mAb MEST-2 bound to monohexosyl ceramide (CMH)/glucosylceramide (GlcCer) from yeast and mycelium forms of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Sporothrix schenckii; mycelia of Aspergillus fumigatus; yeast forms of Candida albicans, Cryptococcus neoformans, C. laurentii, and C. albidus and GlcCer from soybean. Similar results were observed by RIA and immunofluorescence staining. The mAb did not recognize GlcCer from Gaucher's spleen or bovine buttermilk which does not contain 2'-OH fatty acids.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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