Antigenic and functional analysis of a neutralization site of HSV-1 glycoprotein D. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Antigenic and functional analysis of a neutralization site of HSV-1 glycoprotein D.

Dataset

identifier:	1384752
description:	epitope description:S165 antigen name:Envelope glycoprotein D host organism:Mus musculus antibody name:DL11
aggregation:	instance of dataset
availability:	available
primaryPublications:	2154881

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1004975
landingPage:	http://www.iedb.org/assay/1384752

Publication

type:	Literature
publicationVenue:	Virology
dates:	1990

Dimension

study type:	b cell assays
subject species:	Herpes simplex virus (type 1 / strain Patton)

Person

fullName:	M I Muggeridge T T Wu D C Johnson J C Glorioso R J Eisenberg G H Cohen

Data Type

method:	western blot

Study

name:	Antigenic and functional analysis of a neutralization site of HSV-1 glycoprotein D.
description:	Cytomplasmic extracts of COS-1 cells transfected with plasmids expressing HSV-1 gD mutants were used to identify conformational binding sites. A single amino acid change at position 140 (Ser to Asn) abrogated reactivity with the mAb DL11 by creating a new N-linked glycosylation site. A combination of mutations at positions 132, 140 and/or 216 was found to be lethal to the virus.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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