Analyses of the antigenicity of influenza haemagglutinin at the pH optimum for virus-mediated membrane fusion.

Title:

Analyses of the antigenicity of influenza haemagglutinin at the pH optimum for virus-mediated membrane fusion.

identifier:	1403372
description:	epitope description:D63 antigen name:Hemagglutinin host organism:Mus musculus BALB/c antibody name:HC45
aggregation:	instance of dataset
availability:	available
primaryPublications:	6192202

study type:	b cell assays
subject species:	Influenza A virus (A/X-31(H3N2))

fullName:	R S Daniels A R Douglas J J Skehel D C Wiley

method:	biological activity

name:	Analyses of the antigenicity of influenza haemagglutinin at the pH optimum for virus-mediated membrane fusion.
description:	This key residue was identified by sequence analysis of viral escape mutants selected by a monoclonal antibody. Residue Asp63, in site E, was shown to be critical for the binding of mAb HC45 to the viral hemagglutinin by selection of a viral variant with an Asp63 to Asn substitution. Variant virus HA protein did not block the mAb in a hemagglutination inhibition assay. Binding of mAb HC45 to the virus was independent of the pH-dependent conformation of HA.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.