Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1004997 |
| landingPage: |
http://www.iedb.org/assay/1410242 |
| type: |
Literature
|
| publicationVenue: |
Nat Biotechnol
|
| dates: |
2007
|
| study type: | b cell assays |
| subject species: | Clostridium botulinum A 2 |
| fullName: |
Consuelo Garcia-Rodriguez
Raphael Levy
Joseph W Arndt
Charles M Forsyth
Ali Razai
Jianlong Lou
Isin Geren
Raymond C Stevens
James D Marks
|
| method: |
binding assay
|
| name: |
Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin.
|
| description: |
AR2 scFv was obtained by selecting yeast-displayed mutants of the humanized C25 mAb by increasing the affinity for BoNT/A1. AR2 binds BoNT/A1 with very high affinity and exhibits the highest level of in vivo neutralization, but has minimal neutralizing activity for BoNT/A2. The residues involved in this functional epitope were identified by mutational analysis of the energetic contribution of the individual BoNT/A2 residues. The IgG ka to BoNT/A1 is 3.7x106 M-1 s-1 and to BoNT/A2 is 8.31x105 M-1 s-1.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |