Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat.

Dataset

identifier:	1418856
description:	epitope description:VVGDEGRSVFDD antigen name:Other Fagopyrum esculentum (common buckwheat) protein host organism:Homo sapiens antibody name:patient sera
aggregation:	instance of dataset
availability:	available
primaryPublications:	15310064

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1006009
landingPage:	http://www.iedb.org/assay/1418856

Publication

type:	Literature
publicationVenue:	J Plant Physiol
dates:	2004

Dimension

study type:	b cell assays
subject species:	Fagopyrum esculentum

Person

fullName:	Hiroyuki Yoshioka Tsuyoshi Ohmoto Atsuo Urisu Yoshinori Mine Taiji Adachi

Data Type

method:	western blot

Study

name:	Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat.
description:	The common buckwheat (F. esculentum Moench) strain BC₆F₂ was established by 6 backcrosses and one selfing following interspecific hybridization between common buckwheat (cv. Miyazakizairai) and F. homotropicum Ohnishi. Pooled serum IgE from 8 patients with buckwheat allergy reacted with the epitope by a SPOT assay. The core epitope sequence was deduced to be residues 87-92 (EGRSVF) through study of overlapping peptides. Single amino acid substitutions with Ala or Met established residue Arg89 as critical for binding. Alanine substitutions of Glu87 or Gly88 increased IgE binding.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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