Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat.

Dataset

identifier:	1418903
description:	epitope description:FQSRDEKERERF antigen name:Other Fagopyrum esculentum (common buckwheat) protein host organism:Homo sapiens antibody name:patient sera
aggregation:	instance of dataset
availability:	available
primaryPublications:	15310064

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1006009
landingPage:	http://www.iedb.org/assay/1418903

Publication

type:	Literature
publicationVenue:	J Plant Physiol
dates:	2004

Dimension

study type:	b cell assays
subject species:	Fagopyrum esculentum

Person

fullName:	Hiroyuki Yoshioka Tsuyoshi Ohmoto Atsuo Urisu Yoshinori Mine Taiji Adachi

Data Type

method:	western blot

Study

name:	Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat.
description:	The common buckwheat (F. esculentum Moench) strain BC₆F₂ was established by 6 backcrosses and one selfing following interspecific hybridization between common buckwheat (cv. Miyazakizairai) and F. homotropicum Ohnishi. Pooled serum IgE from 8 patients with buckwheat allergy reacted with the epitope by a SPOT assay. The core epitope sequence was deduced to be residues 179-188 (SRDEKERERF) through study of overlapping peptides. Single amino acid substitutions with Ala or Met did not establish critical residues in the sequence.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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