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identifier: 1436160
description:
epitope description:PRLKHEQSV
host organism:Equus caballus
antibody name:F19
aggregation:
instance of dataset
availability:
available
primaryPublications: 10921846
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1004330
landingPage: http://www.iedb.org/assay/1436160
type:
Literature
publicationVenue:
J Virol Methods
dates:
2000
study type: b cell assays
subject species:
fullName:
I Birch-Machin
S Ryder
L
P Iniguez
M Marault
L Ceglie
S Zientara
C Cruciere
F Cancellotti
G Koptopoulos
J Mumford
M Binns
N Davis-Poynter
D Hannant
method:
ELISA
name:
Utilisation of bacteriophage display libraries to identify peptide sequences recognised by equine herpesvirus type 1 specific equine sera.
description:
The epitope was obtained by panning a random peptide library with antisera raised against EHV-1. The epitope has homology to EHV-1 glycoprotein G residues 327-335 (PEVAHLRSV). The epitope was selected from a phage display library.
The phage clone containing the epitope was selected by immuno-panning with equine antiserum from a gnotobiotic foal infected with EHV-1 strain RAC-H and confirmed by ELISA to have specific recognition by its selecting serum.

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