Mapping of B cell epitopes in measles virus nucleocapsid protein. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Mapping of B cell epitopes in measles virus nucleocapsid protein.

Dataset

identifier:	1463086
description:	epitope description:VKQSRGEA antigen name:Nucleoprotein host organism:Mus musculus BALB/c antibody name:mAbs 3E1, 6E4 and 11C9
aggregation:	instance of dataset
availability:	available
primaryPublications:	16944047

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1006170
landingPage:	http://www.iedb.org/assay/1463086

Publication

type:	Literature
publicationVenue:	Arch Virol
dates:	2007

Dimension

study type:	b cell assays
subject species:	Measles virus strain Edmonston

Person

fullName:	A Zvirbliene I Kucinskaite I Sezaite D Samuel K Sasnauskas

Data Type

method:	ELISA

Study

name:	Mapping of B cell epitopes in measles virus nucleocapsid protein.
description:	Monoclonal antibodies 3E1, 6E4 and 11C9 raised against the recombinant measles nucleocapsid protein (rMeN) were initially found to react with the 419-525aa fragment of MeN. The epitope of these mAbs was then further mapped to residues 440-447 using synthetic peptides spanning this region in ELISA assay. In a separate experiment, single alanine substitutions of Arg444 and Glu446 were shown to abrogate this reactivity demonstrating the importance of these residues.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.