Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/640 |
| landingPage: |
http://www.iedb.org/assay/7428 |
| type: |
Literature
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| publicationVenue: |
FEMS Immunol Med Microbiol
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| dates: |
2004
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| study type: | b cell assays |
| subject species: | Bacillus anthracis |
| fullName: |
Taia T Wang
Patricia F Fellows
Terrance J Leighton
Alexander H Lucas
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| method: |
biological activity
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| name: |
Induction of opsonic antibodies to the gamma-D-glutamic acid capsule of Bacillus anthracis by immunization with a synthetic peptide-carrier protein conjugate.
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| description: |
This epitope is a nonamer consisting of D-glutamic acid residues linked at the gamma-COOH groups. It is derived from the capsule of Bacillus anthracis, which is a polymer of gamma-D-glutamic acid.
The assay was a flow cytometric opsonophagocytosis assay. RAW 264.7 cells that had taken-up bacilli gave a fluorescent peak two orders of magnitude brighter than background fluorescence. Levels of phagocytosis were expressed as percentage of fluorescence-positive gated RAW 264.7 cells. The level of opsonophagocytosis was reduced by inhibition with both the epitope, and with B. licheniformis poly-glutamic acid. Specificity of antibodies was evaluated using a serum pool prepared by combining serum samples from mice that had received two injections. Fluorescein-labeled heat-killed encapsulated B.anthracis bacilli (Ames strain) were incubated with various dilutions of an antibody followed by addition of RAW 264.7 cells.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
