Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 7432 |
| description: |
epitope description:Y75, F76, P77, I78, K79, K80, P81, N82, N83, N84, K85, I86, L87, L148, L149, N150, K151, L152, D153, D154, T155, E156, N157, A158, S159, A160, Y161, A162, A163, N164, A165, G166, V167, D168, N169, R170, E171, C172, I173, S174, M175
antigen name:Major capsid protein L1
host organism:Mus musculus BALB/c
antibody name:16A
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
15260888 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/366 |
| landingPage: |
http://www.iedb.org/assay/7432 |
| type: |
Literature
|
| publicationVenue: |
BMC Microbiol
|
| dates: |
2004
|
| study type: | b cell assays |
| subject species: | Human papillomavirus type 16 |
| fullName: |
Vanessa A Olcese
Yan Chen
Richard Schlegel
Hang Yuan
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| method: |
immuno staining
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| name: |
Characterization of HPV16 L1 loop domains in the formation of a type-specific, conformational epitope.
|
| description: |
The minimal conformational epitope was not fully identified with certainty. However, based on experimental findings, it was shown that the HPV16 L1 loop domain I and possibly domain II are involved in the binding of monoclonal antibody 16A. Conformational epitope. The position relative to the protein in genbank is 40-52, 113-140.
Binding of a monoclonal antibody to the conformational epitope of HPV16.L1 protein was demonstrated by the expression of wild type and chimeric recombinant L1 in SF9 cells infected by recombinant bacculoviruses. The region targeted by the 16A monoclonal antibody was identified as fragments I and II by exchanging protein fragments in 2 different constructs.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
