Disease-specific B Cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens.

Title:

Disease-specific B Cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens.

Dataset

identifier:	7529
description:	epitope description:VKIDNASPAS host organism:Mus musculus BALB/c antibody name:anti-SARS CoV Ab
aggregation:	instance of dataset
availability:	available
primaryPublications:	15272409

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/738
landingPage:	http://www.iedb.org/assay/7529

Publication

type:	Literature
publicationVenue:	J Infect Dis
dates:	2004

Dimension

study type:	b cell assays
subject species:

Person

fullName:	I-Ju Liu Po-Ren Hsueh Chin-Tarng Lin Chien-Yu Chiu Chuan-Liang Kao Mei-Ying Liao Han-Chung Wu

Data Type

method:	immuno staining

Study

name:	Disease-specific B Cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens.
description:	This author-identified mimotope was panned from a random peptide phage-display library using serum from acute SARS-CoV patients and according to authors bears sequence homology with residues 18-27 of CDS4 of SARS-CoV. The assay confirms that the epitope (mimotope) itself generated antibodies against SARS-CoV using SARS-CoV infected Vero cells for detection of antibody-binding. A competitive immunofluorescence assay (IFA) was used to test the abilitiy of synthetic peptide to compete with SARS-CoV for binding of antibody.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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