Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/852 |
| landingPage: |
http://www.iedb.org/assay/9192 |
| type: |
Literature
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| publicationVenue: |
Virus Res
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| dates: |
2001
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| study type: | b cell assays |
| subject species: |
| fullName: |
S C Wu
C W Lin
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| method: |
ELISA
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| name: |
Neutralizing peptide ligands selected from phage-displayed libraries mimic the conformational epitope on domain III of the Japanese encephalitis virus envelope protein.
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| description: |
This peptide is shown to mimic envelope (E) protein from Japanese encephalitis virus (JEV) in its binding to a neutralizing antibody and by eliciting neutralizing antibodies. 12-mer peptide Phage display library was screened with mAb E3.3 with 3 rounds of biopanning to find peptides able to induce a protective response against JEV.
A slight, but above background reactivity of mAb E3.3 to this phage clone was observed. A similar result was seen in a competitive ELISA for inhibition of binding of the mAb to E protein. A phage particle expressing the epitope also inhibits neutralization of JEV by mAb E3.3.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
