Monoclonal antibody to Shiga toxin 2 which blocks receptor binding and neutralizes cytotoxicity. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Monoclonal antibody to Shiga toxin 2 which blocks receptor binding and neutralizes cytotoxicity.

Dataset

identifier:	9993
description:	epitope description:E76 antigen name:Escherichia virus 933W protein host organism:Mus musculus BALB/c antibody name:Mab VTm1.1
aggregation:	instance of dataset
availability:	available
primaryPublications:	10531220

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/878
landingPage:	http://www.iedb.org/assay/9993

Publication

type:	Literature
publicationVenue:	Infect Immun
dates:	1999

Dimension

study type:	b cell assays
subject species:	Enterobacteria phage 933W

Person

fullName:	H Nakao N Kiyokawa J Fujimoto S Yamasaki

Data Type

method:	western blot

Study

name:	Monoclonal antibody to Shiga toxin 2 which blocks receptor binding and neutralizes cytotoxicity.
description:	The enterobacteria phage is the source of the toxin expressed in E.coli strains such as O157:H7. By testing variants and point mutants of Stx2, it was determined that the glutamic acid at position 56 is critical for the binding of monoclonal antibody to the Stx2 protein. The monoclonal antibody cross-reacted with Stx2 from various strains of E. coli, including 0157 V50, 0157V354, V601, H7 TK051, 019:H22K080, 022H-KY019, and OUT:H21 TK096.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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