Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/935 |
| landingPage: |
http://www.iedb.org/assay/10840 |
| type: |
Literature
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| publicationVenue: |
FEMS Immunol Med Microbiol
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| dates: |
2000
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| study type: | b cell assays |
| subject species: | Pseudomonas aeruginosa Immunotype 4 |
| fullName: |
H E Gilleland
L B Gilleland
J Staczek
R N Harty
A Garcí
a-Sastre
P Palese
F R Brennan
W D Hamilton
M Bendahmane
R N Beachy
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| method: |
ELISA
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| name: |
Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection.
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| description: |
Peptide shown to provide protection to source species challenge in a mouse model of chronic pulmonary infection. No increase in protective efficacy was seen for the combined administration of another epitope (peptide 10) from source species. Swiss-Prot entry corresponds to the precursor protein while the positions given in the manuscript correspond to the mature form. This protein is antigenically conserved in all strains of source species.
Pooled serum from 5 mice immunized with recombinant tobacco mosaic virus (TMV) expressing epitope inserted in coat protein are shown to have high titers (>6400) of antibodies directed against the epitope and the source species, but not source species mutant lacking F protein. In mice infected with a mixture of this virus and a recombinant Influenza virus expressing peptide 10 from source protein, similar results are obtained. No reactivity is seen with the wild type carrier virus.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
