Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/935 |
| landingPage: |
http://www.iedb.org/assay/10855 |
| type: |
Literature
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| publicationVenue: |
FEMS Immunol Med Microbiol
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| dates: |
2000
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| study type: | b cell assays |
| subject species: | Pseudomonas aeruginosa Immunotype 4 |
| fullName: |
H E Gilleland
L B Gilleland
J Staczek
R N Harty
A Garcí
a-Sastre
P Palese
F R Brennan
W D Hamilton
M Bendahmane
R N Beachy
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| method: |
in vivo assay
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| name: |
Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection.
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| description: |
Peptide shown to provide protection to source species challenge in a mouse model of chronic pulmonary infection. No increase in protective efficacy was seen for the combined administration of another epitope (peptide 9) from source species. Swiss-Prot entry corresponds to the precursor protein while the positions given in the manuscript correspond to the mature form. This protein is antigenically conserved in all strains of source species.
20 out of 29 mice immunized with recombinantinfluenza virus expressing epitope inserted in hemmaglutinin protein are shown to have normal (without lesions) lungs upon source species challenge, while 7 out of 29 mice with normal lungs were seen in wild type TMV immunized mice. In mice infected with a mixture of this virus and a recombinant tobacco mosaic virus expressing peptide 9 from source protein, similar results are obtained, with 15 out of 28 mice having normal lungs. Absence of source species in lung tissue correlates with the lack of lesions.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
