Recombinant antibodies containing an engineered B-cell epitope capable of eliciting conformation-specific antibody responses.

Title:

Recombinant antibodies containing an engineered B-cell epitope capable of eliciting conformation-specific antibody responses.

identifier:	14651
description:	epitope description:SKRGPGSGF host organism:Oryctolagus cuniculus New Zealand White
aggregation:	instance of dataset
availability:	available
primaryPublications:	8701592

study type:	b cell assays
subject species:

fullName:	J Cook B H Barber

method:	ELISA

name:	Recombinant antibodies containing an engineered B-cell epitope capable of eliciting conformation-specific antibody responses.
description:	A serine replacement of C155 found in the natural sequence was made in order to avoid unwanted S-S bond formation. Anti peptide and anti source protein antisera were assayed for their recognition of constructs carryng the epitope on a mouse monoclonal IgG molecule, either in the CDR3 or FR3 domains. Both antisera failed to significantly recognize peptide-IgG constructs, regardless of the site of peptide insertion. A slightly better recognition of some constructs was seen in a 1/10 sera dilution but not at 1/100 dilution.

name:	iedb
homePage:	http://www.iedb.org

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