Antigenicity and immunogenicity of phage library-selected peptide mimics of the major surface proteophosphoglycan antigens of Entamoeba histolytica.

Title:

Antigenicity and immunogenicity of phage library-selected peptide mimics of the major surface proteophosphoglycan antigens of Entamoeba histolytica.

Dataset

identifier:	11875
description:	epitope description:RSSGVTASGPSGTHQLELVPTEWWBGRE host organism:Mus musculus BALB/c antibody name:EH5
aggregation:	instance of dataset
availability:	available
primaryPublications:	12102717

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/824
landingPage:	http://www.iedb.org/assay/11875

Publication

type:	Literature
publicationVenue:	Parasite Immunol
dates:	2002

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Helen Melzer Paola Fortugno Erfan Mansouri Franco Felici Alexandra Marinets Gerhard Wiedermann Herwig Kollaritsch Bernd-Ulrich Von Specht Michael Duchê ne

Data Type

method:	ELISA

Study

name:	Antigenicity and immunogenicity of phage library-selected peptide mimics of the major surface proteophosphoglycan antigens of Entamoeba histolytica.
description:	This peptide, along with others sharing the GTHPXL consensus motif, mimics the surface proteophosphoglycans (PPGs, a.k.a. lipophosphoglycan) of Entamoeba histolytica in its reactivity to an anti-PPG monoclonal antibody, EH5. The phage-displayed epitope was selected from a random sequence library by panning with the mAb. The reactivity to the phage was confirmed in ELISA. Also, the membrane preparation of E. histolytica used to generate the mAb competes with the peptide in binding to the mAb.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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