Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Immunological characteristics associated with the protective efficacy of antibodies to ricin. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1113 |
| landingPage: |
http://www.iedb.org/assay/15467 |
| type: |
Literature
|
| publicationVenue: |
J Immunol
|
| dates: |
2004
|
| study type: | b cell assays |
| subject species: |
| fullName: |
Massimo Maddaloni
Corrie Cooke
Royce Wilkinson
Audrey V Stout
Leta Eng
Seth H Pincus
|
| method: |
biological activity
|
| name: |
Immunological characteristics associated with the protective efficacy of antibodies to ricin.
|
| description: |
Phage displayed peptides recognized by a mAb raised against source protein chain A (RAC) share the motif GTXS, and one peptide (GTHSWDHPHHSYC) containing that motif is able to compete mAb-Rac binding. By modelling on RAC structure, 2 possible fits are seen, one with G213, T217 and S222 and the other with G159, T160 and S156. Swiss-prot ID correspond to the ricin precursor, containing Signal peptide, Chain A, linker peptide and Chain B.
The epitope specific mAb 23 effectively inhibits RCA enzymatic activity in a cell-free translation system. No protection is obtained against ricin toxicity on H9 cells or in the hybridoma cells producing this mAb, implying this mAb is not able to neutralize the toxicity nither in the extra or intracellular milieu, even when the avidity and inhibition of enzymatic activity in a cell free assay were high. A protective effect is not obtained in in vivo neutralization assays on the toxin mediated sugar blood level reduction and mortality.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |