Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1119 |
| landingPage: |
http://www.iedb.org/assay/15714 |
| type: |
Literature
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| publicationVenue: |
J Biol Chem
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| dates: |
2005
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| study type: | b cell assays |
| subject species: | Toxoplasma gondii RH |
| fullName: |
Peter J Bradley
Chris Ward
Stephen J Cheng
David L Alexander
Susan Coller
Graham H Coombs
Joe Dan Dunn
David J Ferguson
Sanya J Sanderson
Jonathan M Wastling
John C Boothroyd
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| method: |
immuno staining
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| name: |
Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii.
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| description: |
C1 residue doesn&rsquo
t belong to the natural sequence, it was added to aid in coupling of carrier protein. Protein name stands for rhoptry protein 10. It was identified by TOF-MS of purified rhoptries' proteins, and peptide was synthesized and used for immunization of mice. The immune sera immunofluoresce pattern allowed for source protein identification as a rhoptry protein.
Peptide immune sera labels rhoptries in source species, confirming source protein as a rhoptry protein.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
