Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Mapping of epitopes and structural analysis of antigenic sites in the nucleoprotein of rabies virus. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/992 |
| landingPage: |
http://www.iedb.org/assay/16168 |
| type: |
Literature
|
| publicationVenue: |
J Gen Virol
|
| dates: |
2000
|
| study type: | b cell assays |
| subject species: | Rabies virus RC-HL |
| fullName: |
H Goto
N Minamoto
H Ito
N Ito
M Sugiyama
A Kawai
|
| method: |
western blot
|
| name: |
Mapping of epitopes and structural analysis of antigenic sites in the nucleoprotein of rabies virus.
|
| description: |
Epitope was deduced from epitope mapping studies of overlapping 8 mers using a panel of mAbs raised against antigenic sites I and IV.
The reactivity of mAb 12-2 toward wild-type and chimeric N-proteins from Hep-Flury and RC-HL strains of rabies virus was demonstrated. mAb 12-2, directed against antigenic site I, was equally reactive toward wild-type and chimeric N-protein from both strains of rabies. Conversely, the reactivity of mAb 8-1 (directed against antigenic site IV) indicates that it requires the N-terminus of the RC-HL strain for efficient binding.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |