epitope description:T330, T332, S306, K307

antigen name:Genome polyprotein

host organism:Mus musculus BALB/c

antibody name:8B10, 17C8, 10C5, 11C2

instance of dataset

available

registration not required

Literature

Virology

2005

Melissa D Sá

nchez

Theodore C Pierson

Douglas McAllister

Sheri L Hanna

Bridget A Puffer

Laura E Valentine

Mariam M Murtadha

James A Hoxie

Robert W Doms

ELISA

Characterization of neutralizing antibodies to West Nile virus.

These residues are located on Domain III (aa296-415) of source antigen ectodomain, forming a continuous patch on the solvent-exposed surface.

Wild type E protein expressed in the context of subviral particles (SVP) are recognized by 4 mAbs showing neutralizing activity, while mutant E protein harboring mutations at epitopic residues are not recognized. Mutations at residues K307 and T330 abolish recognition by all the 4 mAbs, while mutation at S306 and T332 abolishes recognition of 3 of the mAbs. All the 4 mAbs are of IgG1 isotype. These mAbs inhibit the binding of each other to SVPs, confirming that they recognize a similar or adjacent epitope. The reactivity against the source protein by western blot was maintained in partial denaturing conditions but lost in denaturing conditions. They also recognize the source protein by immunofluorescence with infected cells and WNV SVPs expressing lineage II E protein, but not other closely related flaviviruses. On the other hand, other antibodies directed against domain III, but without neutralizing activity, are reactive with Saint Louis and Japanese encephalitis viruses, as well as Yellow Fever and Powasan viruses, but not other virus tested (Dengue 2, equine encephalitis, etc).

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