Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1173 |
| landingPage: |
http://www.iedb.org/assay/17594 |
| type: |
Literature
|
| publicationVenue: |
Nat Med
|
| dates: |
2005
|
| study type: | b cell assays |
| subject species: | West Nile virus 3000.0259 |
| fullName: |
Theodore Oliphant
Michael Engle
Grant E Nybakken
Chris Doane
Syd Johnson
Ling Huang
Sergey Gorlatov
Erin Mehlhop
Anantha Marri
Kyung Min Chung
Gregory D Ebel
Laura D Kramer
Daved H Fremont
Michael S Diamond
|
| method: |
surface plasmon resonance (SPR)
|
| name: |
Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus.
|
| description: |
These residues are located on adjacent loops of Domain III (aa296-415) of source antigen ectodomain, forming a continuous patch on the solvent-exposed surface.
Humanized (i.e., with CDR regions grafted onto a human Ab backbone) and chimeric (i.e., with mouse variable and human constant regions) versions of neutralizing murine mAb E16 (shown to recognize recombinant DIII domain of source protein with a KD of 3.4 nM), are also able to bind DIII, with affinities of 21 and 10.8 nM respectively. Affinity of the humanized version is shown to be improved by back mutation of framework residues 49 of VL and 71of VH, up to 7.1 nM (Humanized E16.3).
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |