Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1206 |
| landingPage: |
http://www.iedb.org/assay/17675 |
| type: |
Literature
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| publicationVenue: |
Virology
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| dates: |
2005
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| study type: | b cell assays |
| subject species: | West Nile virus strain 385-99 |
| fullName: |
Li Li
Alan D T Barrett
David W C Beasley
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| method: |
western blot
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| name: |
Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains.
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| description: |
This residues are located on Domain III (aa296-415) of source antigen ectodomain, forming 2 continuous patchs (A365+T332 and L312+K310) on the solvent-exposed surface. Genbank ID and positions correspond to the viral genome polyprotein, in which E protein sequence spans aas 291 to 787.
Binding of mAbs that neutralize strain 385-99 to E proteins from different strains in a non-reducing western blot assay shows that some proteins from strains harboring mutations at the epitopic residues are not bound, while other strains (AUS60, SA89) that are escape mutants and do not harbor the T332 mutation are still recognized, implying that neutralization escape is not related to antibody binding loss. This was confirmed for AUS60 mutations in recombinant protein ELISA.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
