Autoantigenicity of DFS70 is restricted to the conformational epitope of C-terminal alpha-helical domain.

Title:

Autoantigenicity of DFS70 is restricted to the conformational epitope of C-terminal alpha-helical domain.

identifier:	18047
description:	epitope description:TEISLKDS antigen name:PC4 and SFRS1-interacting protein host organism:Homo sapiens antibody name:anti-DFS70 Ab
aggregation:	instance of dataset
availability:	available
primaryPublications:	15501393

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Yasushi Ogawa Kazumitsu Sugiura Akihiro Watanabe Mitoshi Kunimatsu Masaki Mishima Yasushi Tomita Yoshinao Muro

method:	western blot

name:	Autoantigenicity of DFS70 is restricted to the conformational epitope of C-terminal alpha-helical domain.
description:	This linear determinant is deduced from reactivity of overlapping 12-mers in serum of 1 of 59 patients positive for DFS70 antibodies. It was determined that the primary autoantibody determinant is alpha-helical, conformation-dependent, and restricted to amino acids 349-435 of DFS70, but specific residues comprising the epitopic determinant(s) were not refined in this publication. Epitope mapping of linear determinants was performed via peptide array of 12-mer peptides spanning DFS70 residues 326-527. In this experiment, the array of peptides was tested against 59 samples of human sera. Only 1 of the 59 sera tested were reactive against the array. In the one reactive sample, there were a total of 5 reactive spots, all corresponding to this C-terminal epitope.

name:	iedb
homePage:	http://www.iedb.org

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