Combinatorial use of antibodies to secreted mycobacterial proteins in a host immune system-independent test for tuberculosis.

Title:

Combinatorial use of antibodies to secreted mycobacterial proteins in a host immune system-independent test for tuberculosis.

identifier:	18843
description:	epitope description:SSDPAWERNDPT antigen name:Diacylglycerol acyltransferase/mycolyltransferase Ag85B host organism:Gallus gallus antibody name:85BCPL4 (anti-Ag85B)
aggregation:	instance of dataset
availability:	available
primaryPublications:	11427548

study type:	b cell assays
subject species:	Mycobacterium tuberculosis

fullName:	C P Landowski H P Godfrey S I Bentley-Hibbert X Liu Z Huang R Sepulveda K Huygen M L Gennaro F H Moy S A Lesley M Haak-Frendscho

method:	western blot

name:	Combinatorial use of antibodies to secreted mycobacterial proteins in a host immune system-independent test for tuberculosis.
description:	Oligoclonal antibodies raised against purified fusion proteins were used in the assay to measure circulating mycobacterial proteins in the sera of Chilean patients with tuberculosis. Median levels of Ag85B in patients with active smear- or culture-positive pulmonary tuberculosis measured using IgY fractions of anti-Ag85B were 6 to 27 times higher than those in healthy controls. Sera from patients with active pulmonary tuberculosis were significantly more likely to contain significantly elevated levels of circulating immunoreactive Ag85 complex or Ag85B than were sera from healthy controls.

name:	iedb
homePage:	http://www.iedb.org

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