Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1284 |
| landingPage: |
http://www.iedb.org/assay/18991 |
| type: |
Literature
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| publicationVenue: |
J Virol
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| dates: |
2003
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| study type: | b cell assays |
| subject species: | Japanese encephalitis virus CH2195LA |
| fullName: |
Cheng-Wen Lin
Suh-Chin Wu
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| method: |
ELISA
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| name: |
A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interacted with neutralizing-antibody combining sites.
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| description: |
Functional epitopic determinants were determined by binding of neutralizing mAb E3.3 binding to site-directed mutants of JEV. Interactions were confirmed with expression of recombinant Fab E3.3.
Affinity of mAb E3.3 to wild-type and single amino acid substitutions of S331, and D332 within Domain III of Envelope protein was performed Non-conservative mutations resulted in affinities in excess of 100 nM.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
