Mutational analysis of ganglioside GM(1)-binding ability, pentamer formation, and epitopes of cholera toxin B (CTB) subunits and CTB/heat-labile enterotoxin B subunit chimeras.

Title:

Mutational analysis of ganglioside GM(1)-binding ability, pentamer formation, and epitopes of cholera toxin B (CTB) subunits and CTB/heat-labile enterotoxin B subunit chimeras.

Dataset

identifier:	21586
description:	epitope description:A10, A46, E51, K91 antigen name:Cholera enterotoxin subunit B host organism:Mus musculus antibody name:15C11
aggregation:	instance of dataset
availability:	available
primaryPublications:	11854209

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1374
landingPage:	http://www.iedb.org/assay/21586

Publication

type:	Literature
publicationVenue:	Infect Immun
dates:	2002

Dimension

study type:	b cell assays
subject species:	Vibrio cholerae

Person

fullName:	Michael G Jobling Randall K Holmes

Data Type

method:	radio immuno assay (RIA)

Study

name:	Mutational analysis of ganglioside GM(1)-binding ability, pentamer formation, and epitopes of cholera toxin B (CTB) subunits and CTB/heat-labile enterotoxin B subunit chimeras.
description:	Single mutational substitutions in cholera toxin B subunit at positions 51 (E to K) and 91 (K to D) partially abolished the binding to monoclonal antibody 15C11 substitutions at positions 10 (A to V) and 46 (A to V) completely abolished the binding. The conformational epitope was deduced by assays performed using substitution mutants of cholera toxin B subunit (CTB) or hybrid proteins (part CTB, part LT-Ip (the heat-labile enterotoxin from E.coli)).

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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